教授 & 研究员

夏永振

教授
博士/硕士生导师
0532-58631575
个人简介 科研项目 学术论文

教育背景

1. 2009/10-2011/10,德国明斯特大学,分子微生物技术研究所,联合培养博士

2. 2006/09-2012/07,bet365 코리아,bet365 코리아,博士

3. 2002/09-2006/07,bet365 코리아,国家生命科学和技术基地班,学士


工作经历

1. 2019/09- 至今, bet365 코리아“齐鲁青年学者”,bet365 코리아,教授(破格)

2. 2018/09 –2019.08, bet365 코리아,bet365 코리아,副教授

3. 2012/07- 2018.09,bet365 코리아,bet365 코리아,讲师

4. 2012/07- 2014/07,bet365 코리아,化学与化工学院,博士后


科研工作简介

我们聚焦于细菌硫代谢和合成生物学创新,在两个方向的研究都取得了不错成绩。具体学术创新与贡献如下:

1.自养细菌一直被认为是环境硫循环的主要贡献者,但异养代谢细菌在硫循环中的贡献一直未被阐明。我们以异养细菌为对象,首次揭示了其存在多条活跃的硫氧化途径,并对涉及途径中的酶在分类、生理与生化层面提出新认知,创新性提出异养细菌的硫代谢是环境硫元素生物地球化学循环不可或缺的一环。硫烷是细菌硫氧化的关键中间产物,并且硫烷在哺乳动物细胞中的代谢与调控受到了广泛关注,但其在原核细胞中的代谢与功能研究一直是空白。我们首次关注到细菌中硫烷的生理代谢与调控功能。提出了硫烷检测新方法,分析了不同细菌中硫烷分子的含量、代谢途径和代谢稳态特征,并证实了硫烷参与了对铜绿假单胞菌的细菌抗逆、耐药和群体效应等重要生理功能的调控机制。

2.在合成生物学工作中:a)众所周知,DNA克隆和感受态细胞制备与转化是大多数生物学实验室的常规必备技术,我们对这些经典技术进行了系统创新:建立了新的DNA克隆和感受态细胞制备方法:建立的DNA克隆方法,其效率已赶超主流商业化试剂盒的水平,但成本仅合5分钱/反应,是最廉价的DNA克隆方法;成功将化学法制备的大肠杆菌感受态细胞的效价优化到目前已报道的最高水平,制备感受态使用的克隆型菌株也被证实具有最快的生长速率。进一步阐明大肠杆菌DNA短同源重组的新分子机制,为进一步开发更简便克隆方法奠定了基础。b)在中国,痛风是仅次于糖尿病的第二大常见的代谢性疾病,但常见药存在集中度高,副作用强的缺陷。我们提出了可借助优化工程益生菌的尿酸降解能力来治疗痛风的新疗法。工程菌降解尿酸需要氧气参与,但肠道中氧气受限,我们的工作解决了这项缺陷,实现了低氧条件下尿酸的快速降解,进一步借助肠道灌胃和血液注射证实:该菌剂可在1小时内快速缓解高尿酸血症小鼠的血尿酸水平。

5年来,本人已发表署名通讯作者或者第一作者的SCI论文共26篇,专著章节1篇。其中一区5篇,影响因子大于10的有4篇,Nature Index期刊2篇。发表在以ISME J, Nucleic Acids Research,Gut Microbes,Redox Biology,STOTEN,Analytical Chemistry等为代表的主流专业期刊上,2篇年均引用超过10,其中1篇年均引用已超25。共承担国家级科研项目4项,合计到账科研经费近170万。工作受到了业内专家的肯定,相继破格晋升为bet365 코리아教授,荣获bet365 코리아校内齐鲁青年学者称号,入选山东省高层次人才。

课题项目

1.国家自然科学基金面上项目,32370037,探究硫氰酸酶协助硫烷硫参与微生物抗氧化的新活性和新机制,在研,主持;

2.山东省自然科学基金面上项目,ZR2023MC004,新型过氧化物酶的生化性质、反应机制和生理功能的研究,在研,主持;

3.国家重点研发计划,基于成药性特征的微生物天然产物合成生物学创制,在研,子课题负责人,主持;

4.国家自然科学基金面上项目,31870085,一条新型异养细菌H2S氧化途径的调控和代谢机制研究,已结题,主持;

5.国家自然科学基金重大研究计划,近海沉积物中硫氧化主要菌群与关键通路的研究,子课题负责人;已结题,主持;

6.国家自然基金国际(地区)合作与交流项目,31961133015,合成塑料降解转化微生物菌群,子课题负责人;已结题,主持;

7.国家自然科学基金青年项目,31500047,QuikChangeTM定点突变中具有同源末端线性DNA分子形成机制的研究,已结题,主持;

8.山东省博士基金,2015BSE27054,DNA聚合酶的链置换活性在QuikChangeTM定点突变中的作用及新应用,已结题,主持。

近5年以第一或通讯作者发表的与本项目有关的论文列表(时间倒序):

一区代表性论文:

1.Tianqi Wang, Xiaoju Li, Honglei Liu, Huaiwei Liu,Yongzhen Xia*, Luying Xun*,Microorganisms uptake zero-valent sulfur via membrane lipid dissolution of octasulfur and intracellular solubilization as persulfide,Sci Total Environ,2024 Apr 20:922:170504. (5 year IF=9.8,一区)

2.Tianqi Wang, Guomei Zhong, Honglei Liu, Huaiwei Liu,Yongzhen Xia*, Luying Xun*, A common mechanism for rapid transfer of zero-valent sulfur between microbial cells, Sci Total Environ. 2023 Sep 15;891:164461. (5 year IF=9.8,一区)

3.Mingxue Ran, Qingbin Li, Yufeng Xin, Shaohua Ma, Rui Zhao, Min Wang, Luying Xun*,Yongzhen Xia*, Rhodaneses minimize the accumulation of cellular sulfane sulfur to avoid disulfide stress during sulfide oxidation in bacteria, Redox Biology, 2022 Jul; 53: 102345. (5 year IF=11.271,一区)

4.Rui Zhao, Zimai Li, Yuqing Sun, Wei Ge, Mingyu Wang, Huaiwei Liu, Luying Xun,Yongzhen Xia*,EngineeredEscherichia coliNissle 1917 with urate oxidase and an oxygen-recycling system for hyperuricemia treatment,Gut Microbes,2022 Jan-Dec; 14(1):2070391. (5 year IF=11.724,一区)

5.Ran Mingxue, Wang Tianqi, Shao Ming, Chen Zhigang, Liu Huaiwei,Xia Yongzhen*, Xun Luying*,Sensitive Method for Reliable Quantification of Sulfane Sulfur in Biological Samples, Anal Chem. 2019, 17;91(18):11981-11986 (5 year IF=7.528,一区)

6.Xia Yongzhen, Li Kai, Li Jingjing, Wang Tianqi, Gu Lichuan*, Xun Luying*. T5 exonuclease-dependent assembly offers a low-cost method for efficient cloning and site-directed mutagenesis, Nucleic Acids Res., 2019, 47(3):e15 (5 year IF=17.21,一区)

7.Yongzhen Xia, Chuanjuan Lü, Ningke Hou,Yufeng Xin,Jihua Liu, Honglei Liu*, Luying Xun*, Sulfide production and oxidation by heterotrophic bacteria under aerobic conditions. ISME J., 2017, Dec;11(12):2754-2766. (5 year IF=13.214,一区Top)

8.Chao Tang#; Jingjing Tang#; Yuemeng Shen; Menghui Liu; Honglei Liu; Huaiwei Liu; LuyingXun*;Yongzhen Xia*; A sulfide-sensor and a sulfane sulfur-sensor collectively regulate sulfur-oxidation for feather degradation byBacillus licheniformis, Communications Biology, 2023, 6(1): 167-167. (IF=6.548,二区)

二区代表性论文:

9.Guanhua Xuan, Luying Xun,Yongzhen Xia*, MarR family proteins sense sulfane sulfur in bacteria, mLife, 2024 (online).

10.Yuqing Yang, Menghui Liu, Tianqi Wang, Qian Wang, Huaiwei Liu, Luying Xun,Yongzhen Xia*; An Optimized Transformation Protocol forEscherichia coliBW3KD with Supreme DNA Assembly Efficiency; Microbiol Spectr. 2022 Dec 21;10(6):e0249722. (IF=3.7,二区)

11.Qiaoli Yu, Mingxue Ran, Yuping Xin, Huaiwei Liu, Honglei Liu,Yongzhen Xia*, Luying Xun*; The Rhodanese PspE Converts Thiosulfate to Cellular Sulfane Sulfur inEscherichia coli; Antioxidants (Basel). 2023 May 20;12(5):1127. (IF=7.0,二区)

12.Huangwei Xu#, Guanhua Xuan#, Huaiwei Liu, Honglei Liu,Yongzhen Xia*, Luying Xun*, Sulfane Sulfur Is an Intrinsic Signal for the Organic Peroxide Sensor OhrR ofPseudomonas aeruginosa, Antioxidants (Basel),2022 Aug 26;11(9):1667. (5 year IF=7.886,二区Top)

13.Qiaoli Yu, Mingxue Ran, Yuqing Yang, Huaiwei Liu, Luying Xun*,Yongzhen Xia*, Optimization of a Method for Detecting Intracellular Sulfane Sulfur Levels and Evaluation of Reagents That Affect the Levels inEscherichia coli, 2022 Jul; 11(7): 1292., Antioxidants (Basel). (5 year IF=7.886,二区Top)

14.Tianqi Wang, Yuqing Yang, Menghui Liu, Honglei Liu, Huaiwei Liu,Yongzhen Xia*, Luying Xun*,Elemental Sulfur Inhibits Yeast Growth via Producing Toxic Sulfide and Causing Disulfide Stress, Antioxidants (Basel)., 2022 Mar 17;11(3):576. (5 year IF=7.886,二区Top)

15.Yuqing Yang, Qiaoli Yu, Min Wang, Rui Zhao, Huaiwei Liu, Luying Xun,Yongzhen Xia*,Escherichia coliBW25113 Competent Cells Prepared Using a Simple Chemical Method Have Unmatched Transformation and Cloning Efficiencies, Front Microbiol., 2022 Mar 24;13:838698. (5 year IF=6.843,二区Top)

16.Tianqi Wang, Mingxue Ran, Xiaoju Li, Yequn Liu, Yufeng Xin, Honglei Liu, Huaiwei Liu,Yongzhen Xia*, Luying Xun*, The Pathway of Sulfide Oxidation to Octasulfur Globules in the Cytoplasm of Aerobic Bacteria, Appl Environ Microbiol., 2022 Feb 8;88(3): e0194121. (5 year IF=5.632,二区Top)

17.Huangwei Xu, Guanhua Xuan, Huaiwei Liu,Yongzhen Xia*, Luying Xun*,Sulfane Sulfur Is a Strong Inducer of the Multiple Antibiotic Resistance Regulator MarR inEscherichia coli, Antioxidants (Basel)., 2021 Nov 6;10(11):1778. (5 year IF=7.886,二区Top)

18.Guanhua Xuan, Chuanjuan Lv, Huangwei Xu, Kai Li, Huaiwei Liu,Yongzhen Xia*, Luying Xun*, Sulfane Sulfur Regulates LasR-Mediated Quorum Sensing and Virulence inPseudomonas aeruginosaPAO1,Antioxidants (Basel)., 2021 Sep 21;10(9):1498. (5 year IF=7.886,二区Top)

19.Yuqing Yang, Tianqi Wang, Qiaoli Yu, Huaiwei Liu, Luying Xun,Yongzhen Xia*, The pathway of recombining short homologous ends inEscherichia colirevealed by the genetic study, Mol Microbiol., 2021 Jun;115(6):1309-1322. (5 year IF=4.157,二区)

20.Yufeng Xin, Rui Gao, Feifei Cui, Chuanjuan Lü, Honglei Liu, Huaiwei Liu,Yongzhen Xia*, Luying Xun*, The Heterotrophic BacteriumCupriavidus pinatubonensisJMP134 Oxidizes Sulfide to Sulfate with Thiosulfate as a Key Intermediate,Appl Environ Microbiol., 2020 Oct 28;86(22):e01835-20. (5 year IF=5.632,二区Top)

21.Guanhua Xuan, Chuanjuan Lü, Huangwei Xu, Zhigang Chen, Kai Li, Honglei Liu, Huaiwei Liu,Yongzhen Xia*, Luying Xun*, Sulfane Sulfur is an intrinsic signal activating MexR-regulated antibiotic resistance inPseudomonas aeruginosa, Mol Microbiol., 2020 Dec;114(6):1038-1048. (5 year IF=4.157,二区)

22.Liu Huaiwei, Fan Kaili, Li Huanjie, Wang Qingda, Yang Yunyun, Li Kai,Xia Yongzhen*, Luying Xun*,Synthetic Gene Circuits EnableEscherichia coliTo Use Endogenous H2S as a Signaling Molecule for Quorum Sensing, ACS Synth Biol. 2019, 20;8(9):2113-2120.(5 year IF=5.373,二区Top)

23.Kai Li, Yufeng Xin, Guanhua Xuan, Rui Zhao, Huaiwei Liu,Yongzhen Xia*, Luying Xun*.Escherichia coliuses different enzymes to produce H2S and reactive sulfane sulfur from L-cysteine, Front Microbiol., 2019 Feb 20;10:298. (5 year IF=6.843,二区Top)